Mary Clare McCorry was Accepted to AIMBE’s FDA Scholars Program

Mary Clare McCorry was accepted to the American Institute for Molecular and Biological Engineering (AIMBE) FDA Scholars Program at the Center for Devices and Radiological Health (CDRH). This program places distinguished post-doctorates in medical and biological engineering within CDRH to work side-by-side with influential decision makers to inform regulatory policy decisions.

Congrats, Mary Clare!

Bonassar Lab Work on 3D Tissue Printing Featured in BioTechniques

Work from the Bonassar Lab on 3D printing of cartilage was featured in the Tech News segment of a recent issue of the journal BioTechniques. The feature, entitled “Print on Demand,” describes the work of multiple laboratories using 3D printing for rapid, scalable manufacturing of biological tissues. It details the work of the Bonassar Lab in developing biological inks using alginate and collagen to produce cartilage for reconstructive surgery and orthopaedic applications.  See BioTechniques article: PRINT ON DEMAND to learn more about our work!

The Bonassar Group Wins 3 Awards at ORS

Three members of the Bonassar Lab won awards for presentations at the 2017 meeting of the Orthopaedic Research Society in San Diego, CA.  Bonassar lab alum Dr. Eddie Bonnevie won a New Investigator Research Award (NIRA) for his work demonstrating the effects of inhibited cartilage lubrication on chondrocyte apoptosis and mitochondrial dysfunction.  PhD student Liz Feeney won a Poster Teaser Presentation Award for her describing the design and functionality of biomimetic cartilage lubricants.  PhD student Mary Clare McCorry won 2nd place in the Podium Presentation competition for the Meniscus Section for her work on controlling fiber formation in tissue engineered meniscus implants by manipulating the glucose content of culture media. Congratulations to Eddie, Liz, and Mary Clare!

The Bonassar Group Travels to the ORS 2017 Annual Meeting

The Bonassar Group is traveling to the ORS 2017 Annual Meeting to present 6 papers and 10 posters. Mary Clare McCorry is an award semi-finalist for the Meniscus Section, and Dr. Edward Bonnevie is an award semi-finalist for NIRA. Take a look through the meeting program, and come see our work. We hope to see you there!

Rebecca Irwin’s Collaboration with Dr. John Kennedy Results in a Grant from the AANA

Rebecca Irwin and Dr. John Kennedy of HSS were awarded a research grant for a project entitled “The Effect of Platelet Rich Plasma and Tribosupplementation in Osteochondral Lesions Treated with Microfracture: An in vivo Rabbit Model” from the Arthroscopy Association of North America (AANA). This work will test the hypothesis that decreased friction improves integration of repaired cartilage with native host tissue by decreasing shear stresses during repaired cartilage development. If tribosupplemntation aids in cartilage repair, this information could alter the clinical approach to osteochondral lesion repair and directly impact treatment options for patients.

Lab Alum, Dr. Kirk Samaroo, Publishes a Paper in Biotribology

Bonassar Lab alum Dr. Kirk Samaroo recently had his manuscript accepted by the journal Biotribology:

Tunable Lubricin-mimetics for Boundary Lubrication of Cartilage

The paper describes the development of a library of brush-copolymers designed to lubricate cartilage. These new biomaterials mimic structure of the lubricating properties of the glycoprotein lubricin, which serves as the primary boundary lubricant for articular cartilage. The polymers are composed polyethylene glycol (PEG) side chains grafted to a poly(acrylic acid) (pAA) backbone, similar to the oligosaccharides that decorate the core protein lubricin.  When attached to gold surfaces, all of these new brush co-polymers lubricated cartilage, with the most efficient configuration lowering coefficient of friction by a factor of 2. This work was conducted in collaboration with Prof. David Putnam of the Meinig School of Biomedical Engineering at Cornell, Dr. Mingchee Tan, an alum of the Putnam Lab, and Dr. Delphine Gourdon of the University of Ottawa.  Dr. Samaroo is the co-founder of and CSO of Dynamic Boundaries, Inc., an Ithaca-based startup company that is developing biomimetic lubricants for the treatment of arthritis.

Jill Middendorf Publishes a Paper in the Journal of Orthopaedic Research

Jill Middendorf published the following paper in the Journal of Orthopaedic Research:

Mechanical properties and structure-function relationships of human chondrocyte-seeded cartilage constructs after in vitro culture

Tissue engineered cartilage is a widely recognized method for the repair of focal cartilage defects. These cartilage constructs must provide mechanical support and function in the joint. To fully understand the function of cartilage constructs and to meet recommendations provided by the FDA, multiple mechanical properties of engineered cartilage constructs need to be measured. Although many studies have identified the compressive properties, few have examined the frictional and shear properties. This study is the first study to perform three mechanical tests (compression, friction, and shear) on a human tissue engineered cartilage implant. Results show compressive and frictional properties improve with increased construct maturation, while shear properties remain constant. This study suggests the various mechanical and biological properties of tissue engineered cartilage improve at different rates, indicating thorough mechanical evaluation of tissue engineered cartilage is critical to understanding the performance of repaired cartilage.

Dr. Nizeet Aguilar Publishes an Article in Acta Biomaterialia

Bonassar Lab alum Dr. Nizeet Aguilar recently had her manuscript “Customized Biomaterials to Augment Chondrocyte Gene Therapy” accepted by the journal Acta Biomateriala. The paper describes the development of a modified alginate biomaterial designed to bind insulin-like growth factor-I (IGF-I). This material was designed to work synergistically with chondrocytes transfected to overexpress IGF-I, enabling control of both the production and availability of the growth factor.  This combined approach proved highly effective, enhancing matrix production by chondrocytes by almost 20-fold.  This work was conducted collaboration with Drs. Stephen Trippel and Shuiliang Shi, both of the Indiana School of Medicine, where Dr. Aguilar is pursuing her postdoctoral studies.  Congratulations, Nizeet!

Alexander Boys has been awarded an NIH F31 Fellowship

Alexander Boys has been awarded an NIH F31 Predoctoral Fellowship. Congrats, Alex!

Katherine Hudson Publishes an Article in Tissue Engineering

Katherine Hudson publishes an article in Tissue Engineering Part A.

Hypoxic Expansion of Human Mesenchymal Stem Cells Enhances 3D Maturation of Tissue Engineered Intervertebral Discs

Tissue engineered IVDs aim to address current surgical limitations by replacing the degenerated disc with a cell-laden scaffold that integrates into the disc space, in hope of restoring mechanical function. Many studies have shown that culture in low oxygen conditions (hypoxia) improves the differentiation of mesenchymal stem cells (MSCs) and maturation of IVD cells. In this study, we determined the effects of a low oxygen environment during cell expansion on tissue culture plastic prior to 3D culture on TE-IVDs made with human MSCs. Our results show expansion in hypoxia is just as important as hypoxia during 3D culture for the development of TE-IVDs. This approach represents a significant step towards efficient production of a clinically relevant TE-IVD replacement.