Alexander Boys has been awarded an NIH F31 Fellowship

Alexander Boys has been awarded an NIH F31 Predoctoral Fellowship. Congrats, Alex!

Katherine Hudson Publishes an Article in Tissue Engineering

Katherine Hudson publishes an article in Tissue Engineering Part A.

Hypoxic Expansion of Human Mesenchymal Stem Cells Enhances 3D Maturation of Tissue Engineered Intervertebral Discs

Tissue engineered IVDs aim to address current surgical limitations by replacing the degenerated disc with a cell-laden scaffold that integrates into the disc space, in hope of restoring mechanical function. Many studies have shown that culture in low oxygen conditions (hypoxia) improves the differentiation of mesenchymal stem cells (MSCs) and maturation of IVD cells. In this study, we determined the effects of a low oxygen environment during cell expansion on tissue culture plastic prior to 3D culture on TE-IVDs made with human MSCs. Our results show expansion in hypoxia is just as important as hypoxia during 3D culture for the development of TE-IVDs. This approach represents a significant step towards efficient production of a clinically relevant TE-IVD replacement.  

Christopher DiDomenico Publishes an Article in the Journal of Biomechanical Engineering

Christopher DiDomenico publishes an article in the Journal of Biomechanical Engineering.

Cyclic Mechanical Loading Enhances Transport of Antibodies into Articular Cartilage

The goal of this study was to characterize antibody penetration through cartilage tissue under mechanical loading. Mechanical stimulation aids in the penetration of some proteins, but this effect has not characterized molecules such as antibodies (>100 kDa), which may hold some clinical value for treating osteoarthritis (OA). Overall, transport enhancement followed a linear trend with strain amplitude (0.25-5%) and a nonlinear trend with frequency (0.25-2.60 Hz), with maximum enhancement found to be at 5% cyclic strain and 1 Hz, respectively. Regions of highest enhancement of transport within the tissue were associated with the regions of highest interstitial fluid velocity, as predicted from finite-element simulations. To our knowledge, this is the first study to test how mechanical stimulation affects the diffusion of antibodies in cartilage and suggest further study into other important factors regarding macromolecular transport.

Mary Clare McCorry Publishes an Article in Acta Biomaterialia

Mary Clare McCorry publishes an article in the journal of Acta Biomaterialia.

A Model System for Developing Tissue Engineered Meniscal Enthesis

Tissue engineered menisci hold great promise as a treatment alternative however lack a means of integrated fixation to the underlying bone needed. The mechanical stability of the meniscus depends on its connection to the underlying bone by a fibrocartilage to bone transition zone called the meniscal enthesis. In this study we developed a simplified and high throughput enthesis model to test experimental parameters. Our results show that injection molding collagen into tubing loaded with decellularized bone plugs resulted in a scaffold with three regions: bone, bone-collagen, and collagen. This technique can be used to mimic the native enthesis morphology and serves as ideal test platform to generate a model tissue engineered enthesis.

Dr. Edward Bonnevie and Mary Clare McCorry Publish an Article in the Journal of Biotribology

Dr. Edward Bonnevie and Mary Clare McCorry have published an article in the journal of Biotribology.

Mesenchymal Stem Cells Enhance Lubrication of Engineered Meniscus Through Lubricin Localization in Collagen Gels

Effective boundary lubrication is essential to the health of the cartilage within our bodies joints. In this paper, we explore the boundary lubricating properties of engineered meniscus tissue. Specifically, we hypothesized that incorporation of mesenchymal stem cells (MSCs) enhance boundary lubrication through production of lubricin. Lubricin, which is a naturally occurring glycoprotein, is known to reduce boundary friction coefficients within the body. Our results indicated that incorporating MSCs into engineered menisci produced more localized lubricin on the tissue compared to meniscal fibrochondrocytes, and this trended significantly with decreased friction coefficients. This work highlights the importance of assaying frictional properties in engineered tissue, and that cell source is one parameter that can be tuned to facilitate effective lubrication.

Bonassar Lab travels to BMES 2016 Annual Meeting

Six members of the Bonassar Lab had their abstracts accepted for the BMES 2016 Annual Meeting (Benjamin Cohen, Jill Middendorf, Rebecca Irwin, Stephen Sloan, Leanne Iannucci, and Kiara Chan). Visit the Cornell Booth at the BMES Annual Meeting for more information on everyone’s talks and posters. Congrats, everyone!

Puetzer Wins Whitaker

Jenny Puetzer Wins Whitaker International Scholarship Award

Jenny Puetzer, a 5th year PhD student in Biomedical Engineering, was recently awarded a Whitaker International Scholarship.
This award will be used to support her postdoctoral studies under Prof. Molly Stevens in the Department of Materials
at Imperial College in London, UK. The Whitaker International Program sends emerging leaders in U.S. biomedical engineering overseas to undertake a self-designed project that will enhance their careers within the field. Jenny’s project will continue her work in orthopaedic tissue engineering using advanced microscopy techniques to study extracellular matrix assembly. Congratulations to Jenny!

ORS 2013 Annual Meeting

Podium Presentations

Nizeet Aguilar, “Comparison of Ecacy of Endogenous and Exogenous IGF-I in Stimulating Matrix Production in Mature and Neonatal Chondrocytes”

Edward Bonnevie, “IGF-I Facilitates the Formation of a Lubricin-Rich Surface Layer in Engineered Meniscal Tissue”

Kirk Samaroo, “Characterization of binding and lubricating properties of biomimetic boundary lubricants for articular cartilage”

Poster Presentations

Darvin Griffin, “Mechanical Performance of Matrix-Induced Autologous Chondrocyte Implantation (MACI) Grafts in an Equine Model at 53 Weeks”

Jennifer Puetzer, “The Effects of Boundary Conditions on the Development of Circumferential Collagen Organization and Mechanical Anisotropy in Tissue Engineered Menisci”

Katherine Hudson, “Dose-Dependent Response of Tissue Engineered Intervertebral Discs to Dynamic Compressive Loading”

Brandon Borde, “Repair of Rat Annulus Fibrosus Defects Using High Density Collagen Gels In Vivo”

JMS Poster Award

Jorge Mojica-Santiago, a second year PhD student in the Bonassar Laboratory, earned the Second Place Poster Award at the 2nd International Spine Research Symposium in Philadelphia, PA for his paper titled “Characterization of Canine-Sized Alginate/Collagen Tissue-Engineered Intervertebral Discs.” His work describes new techniques to generate implants for the replacement of degenerated spinal discs.  Extending previous work in the Bonassar laboratory, Jorge used MRIs of dog spines to design implants to match the shape and structure of canine spinal discs. Congratulations to Jorge!

Happy Holidays from the Bonassar Lab

Happy Holidays from the Bonassar Lab!

The Bonassar Lab attends the Annual BME Holiday Party at the Johnson Museum.